5IXD
Structure of human JAK1 FERM/SH2 in complex with IFN lambda receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 111.170, 193.200, 72.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.290 - 2.850 |
| R-factor | 0.2521 |
| Rwork | 0.249 |
| R-free | 0.28230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4po6 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.041 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.290 | 2.950 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.055 | 0.829 |
| Number of reflections | 18724 | |
| <I/σ(I)> | 20.1 | 2.3 |
| Completeness [%] | 99.4 | 99.3 |
| Redundancy | 5.1 | 5.2 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.1-0.2 M Ammonium Citrate tribasic pH 7, 5-15% PEG 3350 |
