5IWM
2.5A structure of GSK945237 with S.aureus DNA gyrase and DNA.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-28 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9611 |
| Spacegroup name | P 61 |
| Unit cell lengths | 93.785, 93.785, 413.350 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.990 - 2.500 |
| R-factor | 0.18209 |
| Rwork | 0.180 |
| R-free | 0.22402 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2xcs |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.390 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.120 | 0.325 |
| Number of reflections | 65320 | |
| <I/σ(I)> | 6.6 | 1.9 |
| Completeness [%] | 92.5 | 94.2 |
| Redundancy | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 293 | 13% peg 5000MME, 100mM BisTris, pH 6.3 |
