5INK

Mouse Tdp2 reaction product (5'-phosphorylated DNA)-abasic/THF-Mg2+ complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-01
Detector	RAYONIX MX300-HS
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	54.615, 67.662, 166.745
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.680 - 2.150
Rwork	0.165
R-free	0.20110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gz1
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.230
High resolution limit [Å]	2.150	4.630	2.150
Rmerge	0.125	0.050	0.650
Number of reflections	34435
<I/σ(I)>	8.6
Completeness [%]	98.4	99.3	95.4
Redundancy	6.7	6.5	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	PEG3350, 200mM NaOAc, 20 mM Magnesium formate, 100 mM HEPES