5INK
Mouse Tdp2 reaction product (5'-phosphorylated DNA)-abasic/THF-Mg2+ complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-01 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.615, 67.662, 166.745 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.680 - 2.150 |
Rwork | 0.165 |
R-free | 0.20110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gz1 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.230 |
High resolution limit [Å] | 2.150 | 4.630 | 2.150 |
Rmerge | 0.125 | 0.050 | 0.650 |
Number of reflections | 34435 | ||
<I/σ(I)> | 8.6 | ||
Completeness [%] | 98.4 | 99.3 | 95.4 |
Redundancy | 6.7 | 6.5 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | PEG3350, 200mM NaOAc, 20 mM Magnesium formate, 100 mM HEPES |