5IL0
Crystal structural of the METTL3-METTL14 complex for N6-adenosine methylation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-09-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9200 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 101.695, 101.695, 116.478 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.272 - 1.882 |
R-factor | 0.1763 |
Rwork | 0.175 |
R-free | 0.20500 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.879 |
Data scaling software | HKL-2000 |
Phasing software | SHELXDE |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.880 |
Number of reflections | 50152 |
<I/σ(I)> | 37.5 |
Completeness [%] | 99.9 |
Redundancy | 14.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 291 | PEG 8000, sodium citrate |