5IFS

Quantitative interaction mapping reveals an extended ubiquitin regulatory domain in ASPL that disrupts functional p97 hexamers and induces cell death

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-04-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.918
Spacegroup name	P 1 21 1
Unit cell lengths	69.844, 132.914, 96.121
Unit cell angles	90.00, 110.15, 90.00

Refinement procedure

Resolution	33.770 - 2.460
R-factor	0.20352
Rwork	0.201
R-free	0.25061
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cf3
RMSD bond length	0.006
RMSD bond angle	1.096
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	33.800		2.520
High resolution limit [Å]	2.460	11.000	2.460
Rmerge	0.050	0.011	0.461
Number of reflections	58583
<I/σ(I)>	16.72	61.05	2.09
Completeness [%]	97.9	90.2	93.2
Redundancy	2.5		2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	23% PEG3350, 0.3M LiSO4, 0.1M Hepes pH 7.0