5IES
Crystal structure of VRC01c-HuGL2 Fab from an HIV-1 naive donor in complex with with a germline-targeting gp120 engineered outer domain eOD-GT8 at 2.16 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2015-05-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 102.553, 138.604, 147.896 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.448 - 2.160 |
R-factor | 0.1906 |
Rwork | 0.189 |
R-free | 0.21430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jpk |
RMSD bond length | 0.006 |
RMSD bond angle | 0.782 |
Data reduction software | HKL-2000 (v706e) |
Data scaling software | HKL-2000 (v706e) |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.450 | 2.193 |
High resolution limit [Å] | 2.154 | 2.160 |
Rmerge | 0.665 | |
Number of reflections | 52709 | |
<I/σ(I)> | 16.2 | 1.9 |
Completeness [%] | 92.5 | 89.6 |
Redundancy | 3.9 | 3.5 |
CC(1/2) | 0.902 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293.15 | 0.1 M citric acid (pH 4.0), 20% (w/v) PEG6000 with an overall pH 5.0 of the solution, cryo-protected with 25% glycerol |