5IEI
X-ray crystallographic structure of a high affinity IGF2 antagonist (Domain11 AB5 RHH) based on human IGF2R domain 11
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2015-01-24 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.120, 48.560, 62.550 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.360 - 2.800 |
R-factor | 0.22479 |
Rwork | 0.222 |
R-free | 0.24908 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gp0 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.745 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.360 | 2.860 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.200 | 1.200 |
Number of reflections | 3414 | |
<I/σ(I)> | 6.4 | 1.35 |
Completeness [%] | 99.8 | 100 |
Redundancy | 6.2 | 6.4 |
CC(1/2) | 0.989 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 2.0 M Ammonium Sulphate, 0.1M Bis-(2-hydroxyethyl)imino-tris(hydroxymethyl methane, pH 7.0. |