5ICV
Crystal structure of human NatF (hNaa60) bound to a bisubstrate analogue
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-08-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 41 |
Unit cell lengths | 48.448, 48.448, 148.368 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.564 - 1.530 |
R-factor | 0.1912 |
Rwork | 0.191 |
R-free | 0.20840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lx9 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.284 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.564 | 1.568 |
High resolution limit [Å] | 1.530 | 1.530 |
Rmerge | 0.122 | 0.635 |
Number of reflections | 51410 | |
<I/σ(I)> | 18.62 | |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 13 | 11.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 4% Tascimate pH 4.0 and 12% PEG 3500 |