5I76
Crystal structure of FM318, a recombinant Fab adopted from cetuximab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-30 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.482, 68.037, 97.689 |
| Unit cell angles | 90.00, 101.85, 90.00 |
Refinement procedure
| Resolution | 27.717 - 1.922 |
| R-factor | 0.1677 |
| Rwork | 0.166 |
| R-free | 0.20100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yy8 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.135 |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.720 | 1.960 |
| High resolution limit [Å] | 1.920 | 1.930 |
| Number of reflections | 69631 | |
| <I/σ(I)> | 45.25 | 11.26 |
| Completeness [%] | 98.4 | 97.4 |
| Redundancy | 7.5 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | 20% (w/v) PEG 3000 100 mM Imidazole/ Hydrochloric acid pH 8.0 200 mM Zinc acetate |
