5I6V
Structure of F285S, a Cancer-Associated Mutation of the Oncogenic Phosphatase SHP2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.822, 214.263, 55.472 |
Unit cell angles | 90.00, 96.08, 90.00 |
Refinement procedure
Resolution | 55.160 - 1.870 |
R-factor | 0.2029 |
Rwork | 0.201 |
R-free | 0.23170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2shp |
RMSD bond length | 0.004 |
RMSD bond angle | 0.778 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.160 | 1.937 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmerge | 0.142 | 0.644 |
Number of reflections | 86484 | |
<I/σ(I)> | 8.36 | 1.65 |
Completeness [%] | 99.0 | 98.1 |
Redundancy | 2.5 | 2.6 |
CC(1/2) | 0.971 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 297 | 0.2M Sodium Malonate, pH7.0, 20% w/v PEG3350 |