5I5E
Crystal Structure of Transketolase mutants-H66/261C complex with xylulose-5-phoaphate from Pichia Stipitis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 99.885, 182.084, 98.263 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.839 - 1.620 |
R-factor | 0.1592 |
Rwork | 0.158 |
R-free | 0.18150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gpu |
RMSD bond length | 0.012 |
RMSD bond angle | 1.204 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((1.10_2152: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.680 |
High resolution limit [Å] | 1.620 | 3.490 | 1.620 |
Rmerge | 0.104 | 0.081 | 0.720 |
Total number of observations | 559503 | ||
Number of reflections | 113628 | ||
<I/σ(I)> | 9.9 | ||
Completeness [%] | 99.8 | 99.3 | 100 |
Redundancy | 4.9 | 5.6 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | MES, NaCl, PEG400 |