5I51
Crystal Structure of Transketolase mutant-R356L complex with fructose-6-phoaphate from Pichia Stipitis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-10 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 100.828, 183.248, 98.749 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.424 - 1.540 |
| R-factor | 0.142 |
| Rwork | 0.141 |
| R-free | 0.15890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gpu |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.188 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX ((1.10_2152: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.570 |
| High resolution limit [Å] | 1.540 | 4.180 | 1.540 |
| Rmerge | 0.069 | 0.026 | 0.905 |
| Number of reflections | 134744 | ||
| <I/σ(I)> | 8.5 | ||
| Completeness [%] | 100.0 | 99.7 | 100 |
| Redundancy | 8.1 | 8.1 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | MES, NaCl, PEG400 |
