5I51

Crystal Structure of Transketolase mutant-R356L complex with fructose-6-phoaphate from Pichia Stipitis

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004802	molecular_function	transketolase activity
A	0005634	cellular_component	nucleus
A	0005829	cellular_component	cytosol
A	0006098	biological_process	pentose-phosphate shunt

Functional Information from PDB Data

site_id	AC1
Number of Residues	22
Details	binding site for residue TPP A 701

Chain	Residue
A	ALA30
A	ILE187
A	ILE189
A	HIS261
A	ASP379
A	GLU415
A	PHE442
A	TYR445
A	HIS478
A	F6R702
A	CA703
A	HIS66
A	HOH821
A	HOH881
A	HOH955
A	GLY114
A	PRO115
A	LEU116
A	ASP155
A	GLY156
A	GLU160
A	ASN185

---

site_id	AC2
Number of Residues	15
Details	binding site for residue F6R A 702

Chain	Residue
A	HIS27
A	HIS66
A	HIS100
A	ILE189
A	HIS261
A	SER383
A	PHE439
A	HIS466
A	ASP474
A	HIS478
A	ARG525
A	TPP701
A	HOH889
A	HOH1059
A	HOH1061

---

site_id	AC3
Number of Residues	5
Details	binding site for residue CA A 703

Chain	Residue
A	ASP155
A	ASN185
A	ILE187
A	TPP701
A	HOH881

---

site_id	AC4
Number of Residues	3
Details	binding site for residue PEG A 704

Chain	Residue
A	LYS7
A	GLY278
A	GLU290

---

site_id	AC5
Number of Residues	3
Details	binding site for residue PEG A 705

Chain	Residue
A	GLU85
A	LYS88
A	SER284

---

site_id	AC6
Number of Residues	1
Details	binding site for residue PEG A 706

Chain	Residue
A	GLN328

---

site_id	AC7
Number of Residues	5
Details	binding site for residue PEG A 707

Chain	Residue
A	ALA426
A	GLU457
A	THR515
A	HOH801
A	HOH836

---

Functional Information from PROSITE/UniProt

site_id	PS00801
Number of Residues	21
Details	TRANSKETOLASE_1 Transketolase signature 1. RllaVDavaaanSGHPGapLG

Chain	Residue	Details
A	ARG13-GLY33

---

site_id	PS00802
Number of Residues	17
Details	TRANSKETOLASE_2 Transketolase signature 2. GEDGPTHqPIEtlAhfR

Chain	Residue	Details
A	GLY472-ARG488

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	Active site: {"description":"Proton donor","evidences":[{"source":"PubMed","id":"27578891","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"27578891","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"30155962","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"5I5E","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XQK","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"27578891","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"29243887","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"30155962","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5HJE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5HYV","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5I51","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XPS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XQA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XRV","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XS6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XU2","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"27578891","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"29243887","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"30155962","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5HJE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5HYV","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5I51","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5I5E","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XPS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XQA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XS6","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"27578891","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"30155962","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5HJE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5I51","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XPS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XQA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XRV","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"27578891","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"29243887","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"30155962","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5HJE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5HYV","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5I51","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5I5E","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XPS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XQA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XRV","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XS6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XU2","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"27578891","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"30155962","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5I51","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XRV","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"27578891","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"30155962","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5XPS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XRV","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"27578891","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"29243887","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"30155962","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5I51","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XRV","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XS6","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"27578891","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5HJE","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5HYV","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5I5E","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XPS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5XQA","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"27578891","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5I51","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	2
Details	Site: {"description":"Important for catalytic activity","evidences":[{"source":"PubMed","id":"27578891","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---