5I50
Structure of OmoMYC bound to double-stranded DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.538, 95.545, 64.944 |
Unit cell angles | 90.00, 105.90, 90.00 |
Refinement procedure
Resolution | 37.945 - 2.701 |
R-factor | 0.229 |
Rwork | 0.227 |
R-free | 0.27940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5i4z |
RMSD bond length | 0.006 |
RMSD bond angle | 0.776 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.770 | 2.830 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.116 | 0.845 |
Number of reflections | 11787 | |
<I/σ(I)> | 11.9 | 2.4 |
Completeness [%] | 99.4 | 100 |
Redundancy | 6.8 | 6.6 |
CC(1/2) | 0.999 | 0.593 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293.15 | MES, magnesium sulfate, potassium chloride, PEG 400 |