5I4Z
Structure of apo OmoMYC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.064 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 65.700, 65.700, 149.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.348 - 1.950 |
| R-factor | 0.1742 |
| Rwork | 0.173 |
| R-free | 0.19410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nkp |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.135 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.350 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.055 | 0.818 |
| Number of reflections | 24551 | |
| <I/σ(I)> | 17.1 | 1.7 |
| Completeness [%] | 99.3 | 92.9 |
| Redundancy | 5.4 | 4.4 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293.15 | MES, potassium sodium tartrate |
