5I4I
Crystal Structure of Transketolase from Pichia Stipitis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-23 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.7 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 101.546, 185.506, 98.876 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.691 - 1.060 |
R-factor | 0.1257 |
Rwork | 0.125 |
R-free | 0.13270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gpu |
RMSD bond length | 0.007 |
RMSD bond angle | 0.999 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((1.10_2152: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.100 |
High resolution limit [Å] | 1.060 | 2.280 | 1.060 |
Rmerge | 0.077 | 0.052 | 0.420 |
Number of reflections | 418461 | ||
<I/σ(I)> | 10.1 | ||
Completeness [%] | 99.7 | 96.9 | 99.9 |
Redundancy | 10.6 | 7.9 | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M MES, 0.1M NaCl, PEG400 |