5I0Q

Structure of human C4b-binding protein alpha chain CCP domains 1 and 2 in complex with the hypervariable region of mutant group A Streptococcus M2 (K65A, N66A) protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 43 3 2
Unit cell lengths	148.598, 148.598, 148.598
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.533 - 2.293
R-factor	0.2055
Rwork	0.203
R-free	0.22430
RMSD bond length	0.009
RMSD bond angle	1.416
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.533	2.370
High resolution limit [Å]	2.290	2.290
Rmerge		1.000
Number of reflections	47421
<I/σ(I)>	46.4	3.22
Completeness [%]	100.0	100
Redundancy	40.5	32.6
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	1.5 M Ammonium Sulfate, 0.1 M Bis-Tris Propane pH 7.0