5I0B
Structure of PAK4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-23 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 62.986, 62.986, 187.111 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.090 |
| R-factor | 0.19901 |
| Rwork | 0.196 |
| R-free | 0.26889 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x4z |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.540 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.210 |
| High resolution limit [Å] | 3.090 | 3.090 |
| Rmerge | 0.123 | 0.563 |
| Number of reflections | 7478 | |
| <I/σ(I)> | 54.3 | 17 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 13 | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.8M sodium potassium tartrate, 0.1M TRIS pH 8.5 |
