5HZW
Crystal structure of the orphan region of human endoglin/CD105 in complex with BMP9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-29 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97938 |
| Spacegroup name | P 64 |
| Unit cell lengths | 211.245, 211.245, 53.164 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.811 - 4.451 |
| R-factor | 0.2888 |
| Rwork | 0.285 |
| R-free | 0.31810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sex 5i04 5i05 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.634 |
| Data reduction software | DIALS (1.dev.176) |
| Data scaling software | Aimless (0.525) |
| Phasing software | PHASER ((2.5.6)) |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.811 | 4.810 |
| High resolution limit [Å] | 4.301 | 4.301 |
| Rmerge | 0.395 | |
| Number of reflections | 9558 | |
| <I/σ(I)> | 3.5 | 0.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.5 | 9.7 |
| CC(1/2) | 0.997 | 0.259 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.1 M AMMONIUM TARTRATE |
