5HZW

Crystal structure of the orphan region of human endoglin/CD105 in complex with BMP9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-29
Detector	PSI PILATUS 6M
Wavelength(s)	0.97938
Spacegroup name	P 64
Unit cell lengths	211.245, 211.245, 53.164
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.811 - 4.451
R-factor	0.2888
Rwork	0.285
R-free	0.31810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3sex 5i04 5i05
RMSD bond length	0.004
RMSD bond angle	0.634
Data reduction software	DIALS (1.dev.176)
Data scaling software	Aimless (0.525)
Phasing software	PHASER ((2.5.6))
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.811	4.810
High resolution limit [Å]	4.301	4.301
Rmerge	0.395
Number of reflections	9558
<I/σ(I)>	3.5	0.6
Completeness [%]	100.0	100
Redundancy	9.5	9.7
CC(1/2)	0.997	0.259

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1.1 M AMMONIUM TARTRATE