5HZI

Crystal structure of photoinhibitable Intersectin1 containing C450M mutant LOV2 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99998
Spacegroup name	P 1 21 1
Unit cell lengths	62.060, 111.530, 88.330
Unit cell angles	90.00, 90.63, 90.00

Refinement procedure

Resolution	47.153 - 2.600
R-factor	0.2403
Rwork	0.238
R-free	0.28460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ki1 2wkq
RMSD bond length	0.002
RMSD bond angle	0.510
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.070	0.548
Number of reflections	36823
<I/σ(I)>	17.9	3.6
Completeness [%]	99.4	99.2
Redundancy	6.6	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1 M HEPES pH 7.5, 9% (w/v) PEG 8000, 9% (v/v) ethylene glycol