5HYU

Structure of human C4b-binding protein alpha chain CCP domains 1 and 2 in complex with the hypervariable region of group A Streptococcus M2 protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-08
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.984
Spacegroup name	P 43 3 2
Unit cell lengths	148.313, 148.313, 148.313
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.971 - 2.561
R-factor	0.2091
Rwork	0.208
R-free	0.22290
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.355
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.600
High resolution limit [Å]	2.560	2.560
Rmerge	0.180	1.000
Number of reflections	18572
<I/σ(I)>	67.6	4.17
Completeness [%]	100.0	100
Redundancy	42.8	43.1
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	1.5 M Ammonium Sulfate, 0.1 M Bis-Tris Propane
1	VAPOR DIFFUSION	7	293	1.5 M Ammonium Sulfate, 0.1 M Bis-Tris Propane