5HYT
Structure of human C4b-binidng protein alpha chain CCP domains 1 and 2 in complex with the hypervariable region of group A Streptococcus M22 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-22 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.079, 80.348, 152.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 76.470 - 2.540 |
| R-factor | 0.2178 |
| Rwork | 0.213 |
| R-free | 0.27440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hyp |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.485 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.470 | 2.680 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Rmerge | 0.140 | 1.000 |
| Number of reflections | 28415 | |
| <I/σ(I)> | 10.7 | 1.2 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 6.6 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 2 M Ammonium Sulfate, 2% PEG 400, HEPES pH 7.5 |
