5HRO
STRUCTURE OF HIV-1 REVERSE TRANSCRIPTASE In COMPLEX WITH A DNA aptamer and an Alpha-carboxy nucleoside phosphonate inhibitor (alpha-CNP)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-13 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9179 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 90.245, 128.959, 131.049 |
Unit cell angles | 90.00, 101.47, 90.00 |
Refinement procedure
Resolution | 38.520 - 2.750 |
R-factor | 0.2361 |
Rwork | 0.235 |
R-free | 0.26460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5d3g |
RMSD bond length | 0.011 |
RMSD bond angle | 0.745 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.800 |
High resolution limit [Å] | 2.750 | 7.450 | 2.750 |
Rmerge | 0.077 | 0.046 | 0.532 |
Rmeas | 0.088 | ||
Rpim | 0.043 | ||
Total number of observations | 268023 | ||
Number of reflections | 74312 | ||
<I/σ(I)> | 12.5 | ||
Completeness [%] | 95.6 | 91.8 | 87.9 |
Redundancy | 3.6 | 4.3 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 277 | 7% PEG 8000, 100 MM BISTRIS-PROPANE, AMMONIUM SULFATE, 5% GLYCEROL, 5% SUCROSE |