5HRL

The crystal structure of AsfvPolX: 1nt-gap(P) DNA2: dGTP ternary complex.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 21 21 2
Unit cell lengths	124.651, 71.348, 86.922
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.990 - 2.400
R-factor	0.22929
Rwork	0.228
R-free	0.25713
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hr9
RMSD bond length	0.008
RMSD bond angle	1.403
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.460
High resolution limit [Å]	2.400	2.400
Number of reflections	28026
<I/σ(I)>	28.3
Completeness [%]	95.2
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	277	0.1 M BIS-TRIS pH 5.5, 17% w/v PEG 10000, 0.1 M Ammonium acetate