5HPS
System-wide modulation of HECT E3 ligases with selective ubiquitin variant probes: WWP1 and UbV P1.1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-20 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 207.914, 44.908, 60.200 |
| Unit cell angles | 90.00, 96.07, 90.00 |
Refinement procedure
| Resolution | 103.374 - 2.050 |
| R-factor | 0.2114 |
| Rwork | 0.209 |
| R-free | 0.26550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nd7 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.248 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 103.500 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.061 | 0.544 |
| Number of reflections | 34664 | |
| <I/σ(I)> | 12.96 | 2.5 |
| Completeness [%] | 98.7 | 99.3 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | ammonium sulfate, glycerol, PEG 3350 |
