5HPE
Phosphatase domain of PP5 bound to a phosphomimetic Cdc37 substrate peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-19 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.968600 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.314, 65.164, 132.117 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.390 - 2.270 |
| R-factor | 0.1753 |
| Rwork | 0.168 |
| R-free | 0.23590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s95 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.632 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.1.29) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.390 | 2.340 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.164 | 0.657 |
| Number of reflections | 16821 | |
| <I/σ(I)> | 9.5 | 3 |
| Completeness [%] | 92.0 | 86.8 |
| Redundancy | 5.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 18% PEG, 8% ethylene glycol, 0.1M Hepes pH 7.5, 20mM hexamine cobolt chloride |
