5HJQ

Crystal structure of the TBC domain of Skywalker/TBC1D24 from Drosophila melanogaster in complex with inositol(1,4,5)triphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9762
Spacegroup name	P 42 21 2
Unit cell lengths	87.720, 87.720, 97.097
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.230 - 2.300
R-factor	0.2314
Rwork	0.230
R-free	0.25720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hjn
RMSD bond length	0.004
RMSD bond angle	0.806
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.230	2.440
High resolution limit [Å]	2.300	2.300
Rmerge	0.095	0.802
Number of reflections	17353
<I/σ(I)>	18.18
Completeness [%]	99.4
Redundancy	12.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	PEG 1500, sucinate/phosphate/glycine