5HJ3
Crystal structure of host-primed Ebola virus GP, GPcl.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2013-06-23 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.03316 |
Spacegroup name | H 3 |
Unit cell lengths | 193.103, 193.103, 350.320 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.276 - 3.300 |
R-factor | 0.2284 |
Rwork | 0.227 |
R-free | 0.25840 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.699 |
Data reduction software | d*TREK |
Data scaling software | d*TREK (9.9.9.6L) |
Phasing software | PHASER (2.5.4) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.280 | 48.280 | 3.420 |
High resolution limit [Å] | 3.300 | 7.100 | 3.300 |
Rmerge | 0.100 | 0.045 | 0.731 |
Total number of observations | 286816 | 28303 | 28668 |
Number of reflections | 73208 | ||
<I/σ(I)> | 7 | 21.9 | 1.3 |
Completeness [%] | 99.9 | 99.5 | 100 |
Redundancy | 3.89 | 3.85 | 3.88 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 293 | 25% PEG 550 MME, 10% 2-propanol, 5% ethylene glycol, 100 mM sodium acetate pH 4.7, and 100 mM calcium chloride. |