5HJ3

Crystal structure of host-primed Ebola virus GP, GPcl.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	80
Detector technology	PIXEL
Collection date	2013-06-23
Detector	PSI PILATUS 6M
Wavelength(s)	1.03316
Spacegroup name	H 3
Unit cell lengths	193.103, 193.103, 350.320
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.276 - 3.300
R-factor	0.2284
Rwork	0.227
R-free	0.25840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.699
Data reduction software	d*TREK
Data scaling software	d*TREK (9.9.9.6L)
Phasing software	PHASER (2.5.4)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.280	48.280	3.420
High resolution limit [Å]	3.300	7.100	3.300
Rmerge	0.100	0.045	0.731
Total number of observations	286816	28303	28668
Number of reflections	73208
<I/σ(I)>	7	21.9	1.3
Completeness [%]	99.9	99.5	100
Redundancy	3.89	3.85	3.88

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.7	293	25% PEG 550 MME, 10% 2-propanol, 5% ethylene glycol, 100 mM sodium acetate pH 4.7, and 100 mM calcium chloride.