5HDH
Crystal structure of human TLR8 with an uncleaved Z-loop
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-01 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0000 |
Spacegroup name | H 3 2 |
Unit cell lengths | 171.520, 171.520, 301.333 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.020 - 2.600 |
R-factor | 0.20756 |
Rwork | 0.206 |
R-free | 0.23944 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.770 |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 45.020 |
High resolution limit [Å] | 2.600 |
Number of reflections | 49967 |
<I/σ(I)> | 17.3 |
Completeness [%] | 100.0 |
Redundancy | 19.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | ammonium sulfate, sodium acetate, glycerol |