5H3E
Crystal structure of mouse isocitrate dehydrogenases 2 K256Q mutant complexed with isocitrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2014-12-07 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 |
Unit cell lengths | 58.150, 58.150, 206.915 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.320 - 2.210 |
R-factor | 0.211 |
Rwork | 0.209 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4l04 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.080 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.300 | 2.340 |
High resolution limit [Å] | 2.210 | 2.210 |
Number of reflections | 37204 | |
<I/σ(I)> | 16 | 3 |
Completeness [%] | 94.5 | 71.7 |
Redundancy | 4.7 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 293 | 0.1 M ammonium formate, 24%(w/v) PEG 3350 |