5H3A
Structural analysis of KSHV thymidylate synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-05-22 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9797 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.410, 79.539, 67.283 |
Unit cell angles | 90.00, 116.24, 90.00 |
Refinement procedure
Resolution | 19.917 - 2.400 |
R-factor | 0.1812 |
Rwork | 0.178 |
R-free | 0.23220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5h38 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.330 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.365 | 2.400 |
Number of reflections | 23609 | |
<I/σ(I)> | 28.4 | 2.7 |
Completeness [%] | 98.7 | 98.5 |
Redundancy | 4.1 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 300 | potassium phosphate (monobasic), PEG 8K, glycerol |