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5H1D

Crystal structure of C-terminal of RhoGDI2

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRF BEAMLINE BL17U
Synchrotron siteSSRF
BeamlineBL17U
Temperature [K]100
Detector technologyCCD
Collection date2015-04-05
DetectorADSC QUANTUM 315r
Wavelength(s)0.979
Spacegroup nameP 43 21 2
Unit cell lengths55.020, 55.020, 96.800
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution36.340 - 1.494
R-factor0.1564
Rwork0.154
R-free0.20390
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1rho
RMSD bond length0.004
RMSD bond angle0.709
Data reduction softwareiMOSFLM
Data scaling softwareSCALA (3.3.21)
Phasing softwarePHASER
Refinement softwarePHENIX (1.10_2155)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]47.83336.3401.570
High resolution limit [Å]1.4944.7201.494
Rmerge0.0510.502
Number of reflections24846
<I/σ(I)>18.111.71.3
Completeness [%]99.995.5100
Redundancy13.610.713.8
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP29330% PEG 1500

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