5H1D
Crystal structure of C-terminal of RhoGDI2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 55.020, 55.020, 96.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.340 - 1.494 |
R-factor | 0.1564 |
Rwork | 0.154 |
R-free | 0.20390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rho |
RMSD bond length | 0.004 |
RMSD bond angle | 0.709 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA (3.3.21) |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.833 | 36.340 | 1.570 |
High resolution limit [Å] | 1.494 | 4.720 | 1.494 |
Rmerge | 0.051 | 0.502 | |
Number of reflections | 24846 | ||
<I/σ(I)> | 18.1 | 11.7 | 1.3 |
Completeness [%] | 99.9 | 95.5 | 100 |
Redundancy | 13.6 | 10.7 | 13.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30% PEG 1500 |