5GZW

Crystal structure of AmpC BER adenylylated by acetyl-AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-04
Detector	ADSC QUANTUM 270
Wavelength(s)	0.98000
Spacegroup name	P 21 21 21
Unit cell lengths	61.480, 65.039, 177.271
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.678 - 1.489
R-factor	0.2121
Rwork	0.211
R-free	0.23710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c3b
RMSD bond length	0.007
RMSD bond angle	1.167
Data scaling software	HKL-2000
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.520
High resolution limit [Å]	1.489	1.490
Number of reflections	115016
<I/σ(I)>	32
Completeness [%]	98.3
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	5.5	295	0.2M Lithium sulfate, 0.1M Bis-tris pH 5.5, 25% polyethylene glycol 3500