5GVI
Zebrafish USP30 in complex with Lys6-linked diubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-23 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 65 |
Unit cell lengths | 130.270, 130.270, 59.881 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.083 - 1.870 |
R-factor | 0.1779 |
Rwork | 0.176 |
R-free | 0.20600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3i3t |
RMSD bond length | 0.006 |
RMSD bond angle | 1.022 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.900 |
High resolution limit [Å] | 1.870 | 1.870 |
Number of reflections | 47938 | |
<I/σ(I)> | 42.8 | 2.38 |
Completeness [%] | 99.6 | 99.6 |
Redundancy | 7.9 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 90 mM Tris-HCl, 180 mM ammonium acetate, 23% PEG3350, 10 mM TCEP |