5GVI

Zebrafish USP30 in complex with Lys6-linked diubiquitin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Ubiquitin carboxyl-terminal hydrolase 30	polymer	444	50492.8	1	UniProt (A0A0R4ILB8) In PDB	Danio rerio (Zebrafish)
2	B	ubiquitin	polymer	76	8604.8	1	UniProt (P62983) Pfam (PF00240) In PDB	Mus musculus (Mouse)
3	C	ubiquitin	polymer	72	8192.4	1	UniProt (P62983) Pfam (PF00240) In PDB	Mus musculus (Mouse)
4	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
5		water	water		18.0	123	Chemie (HOH)

Sequence modifications

A: 61 - 501 (UniProt: A0A0R4ILB8)

PDB	External Database	Details
Ser 58	-	expression tag
His 59	-	expression tag
Met 60	-	expression tag
Ala 73	Cys 73	engineered mutation
Val 127	Asp 127	engineered mutation

B: 1 - 76 (UniProt: P62983)

PDB	External Database	Details
Arg 6	Lys 6	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	67290.0
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	67355.4

*Water molecules are not included.