5GUL
Crystal structure of Tris/PPix2/Mg2+ bound form of cyclolavandulyl diphosphate synthase (CLDS) from Streptomyces sp. CL190
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2012-10-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.851, 61.661, 82.242 |
| Unit cell angles | 74.00, 84.54, 86.04 |
Refinement procedure
| Resolution | 55.040 - 1.730 |
| R-factor | 0.2187 |
| Rwork | 0.216 |
| R-free | 0.27848 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5guk |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.895 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.040 | 1.760 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.083 | 0.434 |
| Number of reflections | 92115 | |
| <I/σ(I)> | 18.1 | |
| Completeness [%] | 85.9 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100mM HEPES NaOH pH 7.0, 24% PEG 2000 |
