5GS2
Crystal structure of diabody complex with repebody and MBP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2013-11-06 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97933 |
Spacegroup name | H 3 2 |
Unit cell lengths | 278.835, 278.835, 132.322 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.107 - 3.592 |
R-factor | 0.2221 |
Rwork | 0.217 |
R-free | 0.27760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3pgf 3rfj 4j8r |
RMSD bond length | 0.013 |
RMSD bond angle | 1.681 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.730 |
High resolution limit [Å] | 3.600 | 3.600 |
Number of reflections | 22895 | |
<I/σ(I)> | 20.7 | |
Completeness [%] | 100.0 | |
Redundancy | 10.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 296 | 0.1 M Sodium acetate pH 4.2, 2 M Ammonium sulfate |