5GRM

Crystal structure of rat STING in complex with cyclic GMP-AMP with 2'5'and 3'5'phosphodiester linkage(2'3'-cGAMP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-09
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.1000
Spacegroup name	P 41 21 2
Unit cell lengths	77.200, 77.200, 152.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.378 - 1.550
R-factor	0.1815
Rwork	0.181
R-free	0.19290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4loj
RMSD bond length	0.035
RMSD bond angle	2.327
Data reduction software	XDS
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.600
High resolution limit [Å]	1.550	1.550
Rmerge		0.572
Number of reflections	67310	6032
<I/σ(I)>	20.12	3.45
Completeness [%]	99.8	100
Redundancy	7.14	7.14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	1.8M (NH4)2SO4, 0.1M HEPES pH7.0