5GP7
Structural basis for the binding between Tankyrase-1 and USP25
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-14 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97776 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.506, 59.242, 39.995 |
Unit cell angles | 90.00, 100.68, 90.00 |
Refinement procedure
Resolution | 39.303 - 1.502 |
R-factor | 0.1743 |
Rwork | 0.172 |
R-free | 0.20720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3twu |
RMSD bond length | 0.008 |
RMSD bond angle | 0.956 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 25690 | |
<I/σ(I)> | 10.93 | |
Completeness [%] | 98.7 | |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 100 mM HEPES/sodium hydroxide, 200 mM sodium chloride, 20% (w/v) PEG 6000 |