5GON
Structures of a beta-lactam bridged analogue in complex with tubulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-17 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97845 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 104.507, 156.463, 181.838 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 118.600 - 2.480 |
| R-factor | 0.208 |
| Rwork | 0.205 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.563 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 118.600 | 2.570 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Number of reflections | 98970 | |
| <I/σ(I)> | 22 | |
| Completeness [%] | 92.9 | |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 6% polyethylene glycol 4000, 8% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7 |
