5GJE
Three-dimensional reconstruction of human LRP6 ectodomain complexed with Dkk1
EM specimen
| Staining | Yes |
| Staining material | Uranyl acetate |
EM Data Collection
| Microscope | HITACHI H-9500SD |
| Electron Gun | LAB6 |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.8 |
| Detector | TVIPS TEMCAM-F224 (2k x 2k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 21 |
| Software | SPIDER & WEB,SPIDER,EM-Menu,Chimera |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
