5G2N
X-ray structure of PI3Kinase Gamma in complex with Copanlisib
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | PILATUS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.762, 68.336, 106.472 |
| Unit cell angles | 90.00, 95.11, 90.00 |
Refinement procedure
| Resolution | 61.780 - 2.680 |
| R-factor | 0.21512 |
| Rwork | 0.211 |
| R-free | 0.28454 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.909 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.780 | 2.830 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Rmerge | 0.080 | 0.670 |
| Number of reflections | 29372 | |
| Completeness [%] | 96.6 | 97.5 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 19% PEG4000,0.15M (NH4)2SO4, 0.1M TRIS PH7.5 |
