5FYM
Crystal structure of JmjC domain of human histone demethylase UTY in complex with D-2-hydroxyglutarate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-05 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 90.480, 109.340, 117.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.817 - 2.000 |
| R-factor | 0.1949 |
| Rwork | 0.192 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zli |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.011 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | DIMPLE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.820 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.160 | 1.500 |
| Number of reflections | 79206 | |
| <I/σ(I)> | 8.5 | 1.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.8 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 0.1M HEPES PH 7.4, 0.2M TRIMETHYLAMINE N-OXIDE, 15% PEG3350 |
