5FV7
Human Fen1 in complex with an N-hydroxyurea compound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-09-12 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.339, 50.193, 66.945 |
| Unit cell angles | 102.06, 94.01, 90.74 |
Refinement procedure
| Resolution | 65.290 - 2.840 |
| R-factor | 0.23712 |
| Rwork | 0.234 |
| R-free | 0.30021 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.023 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.070 | 2.940 |
| High resolution limit [Å] | 2.840 | 2.840 |
| Rmerge | 0.090 | 0.260 |
| Number of reflections | 12396 | |
| <I/σ(I)> | 6.1 | 2.5 |
| Completeness [%] | 95.4 | 90.7 |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 25% PEG3350, 0.1M MOPS 7.0, 5% 2-PROPANOL, 2% GLYCEROL, pH 7.5 |
