5FUZ
Extending the half-life of a Fab fragment through generation of a humanised anti-Human Serum Albumin (HSA) Fv domain: an investigation into the correlation between affinity and serum half-life
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-12-05 |
Detector | DECTRIS PILATUS |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 111.210, 111.210, 89.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.680 |
R-factor | 0.2119 |
Rwork | 0.212 |
R-free | 0.25090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | IN-HOUSE FAB |
RMSD bond length | 0.007 |
RMSD bond angle | 1.421 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.820 |
High resolution limit [Å] | 2.680 | 2.680 |
Rmerge | 0.120 | 0.360 |
Number of reflections | 18151 | |
<I/σ(I)> | 23 | 10.1 |
Completeness [%] | 99.5 | 99.4 |
Redundancy | 21.5 | 22.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 2 M DL-MALIC ACID |