5FUN
Crystal structure of human JARID1B in complex with GSK467
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-06 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 142.842, 142.842, 152.011 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.898 - 2.300 |
R-factor | 0.182 |
Rwork | 0.180 |
R-free | 0.21170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5a1f |
RMSD bond length | 0.003 |
RMSD bond angle | 0.809 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.900 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.110 | 0.110 |
Number of reflections | 41059 | |
<I/σ(I)> | 17.27 | 1.68 |
Completeness [%] | 100.0 | 100 |
Redundancy | 12.9 | 13.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 0.1M HEPES PH 8.0, 0.8M POTASSIUM PHOSPHATE-DIBASIC, 0.8M SODIUM PHOSPHATE MONOBASIC |