5FSO
MTH1 substrate recognition: Complex with a methylaminopyrimidinedione.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-D |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | RIGAKU SATURN 92 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 60.313, 66.143, 36.197 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.200 - 1.670 |
R-factor | 0.185 |
Rwork | 0.184 |
R-free | 0.21220 |
Structure solution method | OTHER |
Starting model (for MR) | NONE |
RMSD bond length | 0.010 |
RMSD bond angle | 1.050 |
Refinement software | BUSTER (2.11.6) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 36.000 |
High resolution limit [Å] | 1.670 |
Rmerge | 0.060 |
Number of reflections | 14832 |
Completeness [%] | 85.0 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 25% (W/V) PEG3350 200MM LITHIUM SULPHATE 100MM SODIUM ACETATE PH4.5 |