5FS8
Crystal structure of the G308E mutant of human apoptosis inducing factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2012-07-07 |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.790, 89.750, 60.330 |
| Unit cell angles | 90.00, 94.93, 90.00 |
Refinement procedure
| Resolution | 89.750 - 1.400 |
| R-factor | 0.16583 |
| Rwork | 0.164 |
| R-free | 0.19247 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m6i |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.541 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.170 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.060 | 0.620 |
| Number of reflections | 102452 | |
| <I/σ(I)> | 9.4 | 1.5 |
| Completeness [%] | 97.1 | 88.7 |
| Redundancy | 3.6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2 M AMMONIUM ACETATE AND 20% POLY-ETHYLENE GLYCOL, pH 7.5 |
