5FQL
Insights into Hunter syndrome from the structure of iduronate-2- sulfatase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-13 |
Detector | ADSC CCD |
Wavelength(s) | 0.9763, 1.475 |
Spacegroup name | P 31 1 2 |
Unit cell lengths | 71.050, 71.050, 285.879 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.525 - 2.300 |
R-factor | 0.1824 |
Rwork | 0.181 |
R-free | 0.20810 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.003 |
RMSD bond angle | 0.614 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.520 | 2.390 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.080 | 0.940 |
Number of reflections | 35738 | |
<I/σ(I)> | 12.4 | 1.9 |
Completeness [%] | 96.2 | 97.7 |
Redundancy | 5.3 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.1 | 0.1 M MES PH=6.1 1.0 M LICL 20% PEG6K |