5FQF
The details of glycolipid glycan hydrolysis by the structural analysis of a family 123 glycoside hydrolase from Clostridium perfringens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.030, 114.790, 134.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 87.370 - 2.150 |
| R-factor | 0.14962 |
| Rwork | 0.147 |
| R-free | 0.19818 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fqe |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.742 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.140 | 0.500 |
| Number of reflections | 72256 | |
| <I/σ(I)> | 10 | 3.8 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 6.7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
